Establishment Of Tissue Culture Technique Towards Transformation Of Oryza Sativa With Omega-3 Desaturase Gene

Abstract

Salinity stress in paddy field had retarded the annual rice productivity influenced by ionic imbalance, physiological damage and biochemical breakdown. Few reports had categorized rice as a salt sensitive crop manifested in slow vegetative growth rate and delayed reproductive stage. In parallel towards high rice market demands, the establishment of new rice variety with high tolerance towards salt is important to overcome the tragic rice agriculture lost due to soil problems. The opening of newly paddy arable land is impossible due to urbanization. Instead of an exploitation of plant genetic sources in conventional crop breeding, advances in molecular breeding should be priority due wider genetic materials. Omega-3-Fatty Acid Desaturase (ω3-FAD) gene from marine microalgae Chorella Vulgaris strain UMT-M1 was employed in this study as a desired insert as the gene clustered as a saline-combat regulator. An initiative in this new variety production require the optimization of powerful tissue culture protocol.