Abstract:
The chemotherapeutic use of antibiotics in aquaculture to control diseases had been
practiced for several decades, which led to several complications such as the
antibiotic resistance in animal’s tissues together with the gastrointestinal imbalance
of the microbial flora in aquatic species. Several probiotics to enhance the
productivity of commonly cultured fish species in Malaysia have been conducted.
However, reliable evidence in related to lactic acid bacteria derived from the
gastrointestinal tract of broiler chicken Gallus gallus as an in-feed probiotic additive
and its effects on African Catfish Clarias gariepinus are limited. This study aims to
isolate lactic acid bacteria from broiler chicken and tilapia Oreochromis niloticus
and evaluate their probitic effects on African Catfish Clarias gariepinus. The
experiment was implemented for 90 days, with a total number of 450 fingerlings of African Catfish, Clarias gariepinus at an average weight of 2.3 ± 0.2g. The fish were
fed with commercial feed twice daily at a ratio of 5% of their body weight.
Antagonistic analysis of isolates was carried out using double-layer agar method to
select candidate probiotics. We also evaluated whether their tolerance
gastrointestinal condition such as ph., bile salt and digestive enzyme, then identified
candidate probiotic using De Man, Rogosa and Sharpe (MRS) agar.
