Isolation Of Lactic Acid Bacteria From Broiler Chicken And Tilapia Oreochromis Niloticus And Asssessment Of Their Probiotic Effects On African Catfish Clarias Gariepinus

Abstract

The chemotherapeutic use of antibiotics in aquaculture to control diseases had been practiced for several decades, which led to several complications such as the antibiotic resistance in animal’s tissues together with the gastrointestinal imbalance of the microbial flora in aquatic species. Several probiotics to enhance the productivity of commonly cultured fish species in Malaysia have been conducted. However, reliable evidence in related to lactic acid bacteria derived from the gastrointestinal tract of broiler chicken Gallus gallus as an in-feed probiotic additive and its effects on African Catfish Clarias gariepinus are limited. This study aims to isolate lactic acid bacteria from broiler chicken and tilapia Oreochromis niloticus and evaluate their probitic effects on African Catfish Clarias gariepinus. The experiment was implemented for 90 days, with a total number of 450 fingerlings of African Catfish, Clarias gariepinus at an average weight of 2.3 ± 0.2g. The fish were fed with commercial feed twice daily at a ratio of 5% of their body weight. Antagonistic analysis of isolates was carried out using double-layer agar method to select candidate probiotics. We also evaluated whether their tolerance gastrointestinal condition such as ph., bile salt and digestive enzyme, then identified candidate probiotic using De Man, Rogosa and Sharpe (MRS) agar.