Inhibitory Effects Of Pandanus Tectorius Fruit Extracts, Tangeretin, And Trans-Ethyl Caffeate On Hmg-Coa Reductase

Abstract

One of the pathways to reduce cholesterol production in the liver is through the inhibition of HMG-Coa reductase (HMGCR) enzyme generally using statins, but it has side effects when consumed for a prolonged period. Therefore, the current perspective study is to look for a natural agent of HMGCR inhibitor to lower cholesterol levels through the inhibitory mechanism of HMGCR involved in synthesizing cholesterol. Thus, the natural agent can be as an alternative drug in treating hypercholesterolemia and preventing atherosclerosis. The objectives of this study were to investigate the cytotoxicity effects on HepG2 cells and to determine inhibitory effects of Pandanus tectorius fruit extracts, tangeretin, and trans-ethyl caffeate on HMHCR. To evaluate the potential of tangeretin as an anti• hypercholesterolemic agent on hypercholesterolemia-induced rats was also the objective of this study. The cytotoxicity evaluation was conducted by MTT assay on HepG2 cells. Inhibitory effects were done using HMGCR in vitro kit (Sigma Aldrich) with pravastatin and simvastatin as controls. The hyperc-holesterolemic effects of tangeretin were investigated in an intervention study with rats.