Abstract:
This study comprises of three main objectives to understand the organ-specific
oxidative stress responses in caged Lates calcarifer. The first objective emphasised
on the responses of the first line-defense of antioxidant system, superoxidase
dismutase (SOD) and catalase (CAT). The second objective focussed on the
responses of Glutathione dependent enzymes, glutathione-s-transferase (GST) and
glutathione reductase (GR). The third objective quantified the protein damages that
had occurred in body organs of L.calcarifer through the response of thiolation (-SH-)
and carbonylation (-CH-). The fish (n=20) were sampled from fish cages in Setiu
Wetland, Semerak and Tumpat. The responses of SOD, CAT, GST and GR were
measured in muscle, gill and liver using enzymatic assays while protein damages
were measured using 1-Dimensional electrophoresis approach through fluorescent
tagging of -SH- and -CH-. The responses of SOD and CAT were consistent with the
most responses in liver (p<0.05) followed by gill and muscle. High SOD responses
were followed with lower CAT responses observed in muscle with opposite
responses exhibited by both gill and liver. The responses of GST and GR in all
organs agreed with the responses observed in SOD and CAT.
