Oxidative Stress Regulation In Lates Calcarifer From Local Aquaculture Farms In The East Coast Of Malaysia

Abstract

This study comprises of three main objectives to understand the organ-specific oxidative stress responses in caged Lates calcarifer. The first objective emphasised on the responses of the first line-defense of antioxidant system, superoxidase dismutase (SOD) and catalase (CAT). The second objective focussed on the responses of Glutathione dependent enzymes, glutathione-s-transferase (GST) and glutathione reductase (GR). The third objective quantified the protein damages that had occurred in body organs of L.calcarifer through the response of thiolation (-SH-) and carbonylation (-CH-). The fish (n=20) were sampled from fish cages in Setiu Wetland, Semerak and Tumpat. The responses of SOD, CAT, GST and GR were measured in muscle, gill and liver using enzymatic assays while protein damages were measured using 1-Dimensional electrophoresis approach through fluorescent tagging of -SH- and -CH-. The responses of SOD and CAT were consistent with the most responses in liver (p<0.05) followed by gill and muscle. High SOD responses were followed with lower CAT responses observed in muscle with opposite responses exhibited by both gill and liver. The responses of GST and GR in all organs agreed with the responses observed in SOD and CAT.