Abstract:
The work presented in this thesis described the development of the biosensors with
optical and electrochemical detections for determination of polycyclic aromatic
hydrocarbons (PAHs) in environmental monitoring of water. This research employs the
enzyme linked immunosorbent-assay (ELISA ) principle, in which an alkaline
phosphatase (AP) and a horseradish peroxidase (HRP) were used as the enzyme labels.
The influence of several parameters such as the concentration of the biocomponents, the suitable organic solvents and the working pH was tested in order to achieve maximal
binding of the antigen-antibody.
