Gene expression study on cadmium stress in Chlorella vulgaris (UMT-M1) by using mRna differential display polymerase chain reaction (dd-pcr)

Abstract

Cadmium is a non-essential metal which is toxic to all organisms and was classified as carcinogen by several agencies. This study utilizes Chlorella vulgaris (UMT-M1) as a model to study cadmium induced stress in cells. C. vulgaris were exposed to 0.26 mg/L and 2.6 mg/L of cadmium and a total of nine cadmium-inducible genes were identified using differential display polymerase chain reaction (DD-PCR). The sizes of isolated DNA fragments ranged from 242 to 1009 bp which was grouped into three categories based on their function.