Isolation and characterisation of heparin binding protein in slipper oyster, Crassostrea iredalei (faustino 1932) and its role in prophenoloxidase-activating system

Abstract

Recognition proteins play an important role in the immunodefense system of invertebrates. Thus, present study aimed to isolate a recognition protein, via Heparin column from Slipper cupped oyster, Crassostrea iredalei haemolymph plasma to elucidate its roles in prophenoloxidase-activation systems (proPO) and to characterise it. The protein was isolated using affinity column chromatography with yield of 8% and a single peak.