Characterization of target-site mutation in 5-Enolpyruvylshikimate-3-Phosphate Synthase (EPSPS) gene from glyphosate-resistant biotype of Eleusine indica (L.) gaertn from Malaysia

Abstract

The population of goosegrass (Eleusine indica), resistant to glyphosate (an EPSPS–inhibiting herbicide) was found widespread in several states in Malaysia. However, the resistance levels in this plants which could lead to the understanding of its resistance mechanisms are not well studied. This study was therefore carried out to investigate different levels of resistance of goosegrass to glyphosate by the identification of mutations in the EPSPS gene. Whole plant bioassay confirmed that 9 out of the 20 populations collected were resistant, six populations were susceptible, while five populations were a mixture of resistant and susceptible biotypes.