Establishment of organogenesis protocol for genetic modification of "yello pitaya' Selenicereus megalanthus (cactaceae)

Abstract

There is a need to improve the existing germplasm of yellow pitaya (Selenicereus megalanthus), particularly for fruit quality and traits. The genetic transformation requires organogenesis and shoot regeneration protocols. Therefore, the in vitro culture of the plant was successfully established from mature seeds. Results showed that 90% of the seeds were germinated. The plantlets require MS basalt medium for growth. MS added with 2 mg/L BAP (6-benzyldadenine) was suitable for organogenesis and production of explant for other purposes