Abstract:
An aquatic plant, Aglaonema simplex has been successfully cultured in - vitro using
shoot tip in MS medium containing 1.0 mg/L of BAP. The optimum sterilization
condition was by using 100% (v/v) of Clorox with 30 minutes of immersion time. The
effect of media (MS medium, B5 medium and AS medium) and cytokinin (BAP, Kinetin
and 2iP) were examined. The best growth was obtained in MS medium containing 5.0
mg/L of BAP. Three types of explants, which were complete plantlet, shoot in
longitudinal section and shoot in cross section were used for induction of shoot
proliferation. The highest number of shoot proliferation was obtained using longitudinal
section, which cultured in MS medium containing 5.0 mg/L of BAP.
