In vitro culture of aquatyic plant (Aglaonema simplex)

Abstract

An aquatic plant, Aglaonema simplex has been successfully cultured in - vitro using shoot tip in MS medium containing 1.0 mg/L of BAP. The optimum sterilization condition was by using 100% (v/v) of Clorox with 30 minutes of immersion time. The effect of media (MS medium, B5 medium and AS medium) and cytokinin (BAP, Kinetin and 2iP) were examined. The best growth was obtained in MS medium containing 5.0 mg/L of BAP. Three types of explants, which were complete plantlet, shoot in longitudinal section and shoot in cross section were used for induction of shoot proliferation. The highest number of shoot proliferation was obtained using longitudinal section, which cultured in MS medium containing 5.0 mg/L of BAP.