Abstract:
The effects of phenanthrene on Isochrysis galbana were carried out using batch culture and continuous culture techniques. The growth tests were conducted in enriched sea water at 28.0 ± 1.0 °C, pH of 8.1 ± 0.5, salinity 30 ± 2 ppt and under continuous light illumination of 45jLimol quanta/m2/s. For batch culture, growth was estimated by increase in cell number with time. Extent of growth inhibition was influenced by concentration of phenanthrene and duration of exposure. Phenanthrene concentration of 5 mg/1 and 7 mg/1 temporarily inhibited the growth of cells and lethal effect was observed for concentrations higher than 7 mg/1. The IC50 value (the concentration of phenanthrene that causes 50% inhibition in growth of Isochrysis galbana relative to control) determined by batch culture technique was 3.58 mg/1.