Microprogation of Yam (Dioscorea alata) variety in Malaysia by tissue culture technique

Abstract

The objective of this study was to optimize the sterilization technique for shoot tip, axillary bud and tuber explants of Yams for tissue culture. An aseptic technique for clean planting material is required since Yams are always affected by pathogen, viruses and fungus. The sterilization agent used in this study was Clorox which contained 5.25% (v/v) Natrium Hypochorite. The Clorox concentration for axillary bud and shoot tips treatment was started from 10% until 20% and immersion time in Clorox was from 10 minutes to 30 minutes.