Abstract:
Holothuria atra had been collected at coastal area of Bi dong Island in order to isolate and
identify the associated bacteria as well as determine the sugar components in the
polysaccharides produced by polysaccharides producing bacterium. Five bacteria were
isolated from different parts of this animals namely as Gl.1, Gl.5, Gl.7, Gl.12 and
Gl.14. Identification has been performed based on their morphology, basic biochemical
test and identification kit. The identification indicated G 1.1 as Shewanella putrefaciens,
Gl.5 as Sphingomonas paucimobilis, Gl.12 as Acinetobacter calcoaceticus, Gl.14 as
Brevundimonas diminuta and G 1. 7 as a Gram-positive bacteria Peptostreptococcus
asaccharolitycus. All bacteria successfully produced polysaccharides. The
polysaccharides that had been described were tested using Paper Chromatography (PC)
composed of monosaccharides mainly glucose, galactose, mannose, maltose, raffinose
and rhamnose.
