Taxonomical studies of polysaccharide producing bacteria from sea cucumber (holoturia atra)

Abstract

Holothuria atra had been collected at coastal area of Bi dong Island in order to isolate and identify the associated bacteria as well as determine the sugar components in the polysaccharides produced by polysaccharides producing bacterium. Five bacteria were isolated from different parts of this animals namely as Gl.1, Gl.5, Gl.7, Gl.12 and Gl.14. Identification has been performed based on their morphology, basic biochemical test and identification kit. The identification indicated G 1.1 as Shewanella putrefaciens, Gl.5 as Sphingomonas paucimobilis, Gl.12 as Acinetobacter calcoaceticus, Gl.14 as Brevundimonas diminuta and G 1. 7 as a Gram-positive bacteria Peptostreptococcus asaccharolitycus. All bacteria successfully produced polysaccharides. The polysaccharides that had been described were tested using Paper Chromatography (PC) composed of monosaccharides mainly glucose, galactose, mannose, maltose, raffinose and rhamnose.