Isolation and identification of polysaccharide producing bacterium from marine sponge (haliclona sp)

Abstract

This investigation was performed to isolate and to identify the sugar compositions of polysaccharides from marine bacteria isolate from marine sponge, Haliclona sp. which was collected from Bidong's Island. Six marine bacteria were isolated Oligella urethalis, Pantoea agglomerans, and two bacteria from genus Enterobacter; Enterobacter sakazaki and Enterobacter intermedium and two bacteria from genus Citrobacter; Citrobacter freundii and Citrobacter koseri. All bacteria isolated were bacteria gram negative and successfully produce polysaccharides. Sugar compositions in polysaccharides were identified using paper chromatography method. The sugar compositions determined in the polysaccharides were mannose, glucose, raffinose, xylose, arabinose and rhamnose.