Abstract:
Twenty pnmers were screened. Four pnmers, OPA4, OPA7, OPAlO and OPA16 showed the clearest bands and polymorphism was choosen for C,yptocoryne ciliata. Vitrification technique was used to obtain the cold storage procedure for C. ciliata. No surviving explant was obtained after being immersed in liquid nitrogen. Preculture, loading, dehydration, unloading and warming upon cold storage of explants were examined. After two to four days of preculture, 30 minutes of loading period, one hour of dehydration in PVS (Plant Vitrification Solution), 20 minutes of unloading treatment and warming in 40 °C for one minutes.