Cryostorage of cryptocoryne ciliata

Chee Pui Yee

dc.contributor.author	Chee Pui Yee
dc.date.accessioned	2017-07-19T03:38:29Z
dc.date.available	2017-07-19T03:38:29Z
dc.date.issued	2005
dc.identifier.uri	http://hdl.handle.net/123456789/6493
dc.description.abstract	Twenty pnmers were screened. Four pnmers, OPA4, OPA7, OPAlO and OPA16 showed the clearest bands and polymorphism was choosen for C,yptocoryne ciliata. Vitrification technique was used to obtain the cold storage procedure for C. ciliata. No surviving explant was obtained after being immersed in liquid nitrogen. Preculture, loading, dehydration, unloading and warming upon cold storage of explants were examined. After two to four days of preculture, 30 minutes of loading period, one hour of dehydration in PVS (Plant Vitrification Solution), 20 minutes of unloading treatment and warming in 40 °C for one minutes.	en_US
dc.language.iso	en	en_US
dc.publisher	Terengganu: Kolej Universiti Sains dan Teknologi Malaysia	en_US
dc.subject	LP 4 FST 1 2005	en_US
dc.subject	Chee Pui Yee	en_US
dc.title	Cryostorage of cryptocoryne ciliata	en_US
dc.type	Working Paper	en_US