Phytochemical study of chemical constituents from quassia indica

Abstract

Quassia lndica is from Simaroubaceae family. The purpose of this study is to isolate and determine the chemical constituents from Quassia indica. Petroleum ether, chloroform and methanol were used in extraction processes. However, only chloroform extract was isolated and further purified. The preliminary tests, which were alkaloid test and cardiac glycoside test that performed to CEOC showed positive results. In this study, thin layer chromatography (TLC) and column chromatography (CC) were used for isolation and purification ofCEOC. According to the chromatograms of GC-FID, two fractions were successfully purified and labeled as C3CE 3 and C3CE 25. The fractionates were characterized and identified using spectroscopic methods, which were Ultra Violet-Visible (UV-Vis) Spectroscopy, Infrared (IR) Spectroscopy and Gas Chromatography-Mass Spectrometry (GC-MS). The suggested compound for C3CE 3 and C3CE 25 were benzoic acid derivative and phenolic derivative compound respectively.