Abstract:
Microalgae are able to assimilate various types of nitrogen. The interaction of nitrogen
on their removal by microalgae should be of great concern when microalgae are used as a biological
treatment in waste water. This study aims to reveal the preference of nitrogenous compounds by
Nannochloropsis sp. The microalgae were cultivated in 900 µM nitrate, 900 µM ammonium, and
900 µM nitrate plus 900 µM ammonium. Nannochloropsis sp. preferred ammonium rather than
nitrate when both compounds were available to the microalgae. Nitrate was utilised in the absence
of ammonium. The uptake rate of ammonium was signifcantly higher than nitrate (p = 0.004).
There was signifcant difference in the growth rate of the microalgal with different nitrogen sources
(p < 0.000). Nannochloropsis sp. grew faster in treatment containing ammonia. However, there
were no signifcant difference in the maximum cell density produced by the cultures in different
nitrogenous nutrients (p = 0.224). Nannochloropsis sp. can be used as a biological treatment for
waste water. The microalgae will frst remove ammonium and remediate ammonia toxicity to most
fsh. Once ammonium has been removed or brought down to a safer level, nitrate can be removed
by the microalgae.
