Abstract:
A study was done to characterize the NiV L gene, and to develop and analyse the transcription process of NiV. In this study, NiV L protein was cloned and the entire
gene was sequenced. The L gene, which consists of 6,735 nucleotides, encoded a
protein of 2,244 amino acids with a calculated molecular weight of 257,202 Da.
Expression of the L gene in yeast, Pichia pastoris showed the predicted molecular
weight of 257 kDa protein.