Somaclonal variation of long term culturing and high cytokinin concentration on Ananas Comosus N36 cultures using RAPD (Random amplified polymerase DNA) analysis

Abstract

Twenty primers were screened for Ananas comosus Var. N36 cultures. Three primer OPA1, OPA4 and OPA 13 which showed clearest band and polymorphism was chosen to detected the somaclonal variation in three generation i.e. (six, seven and eight) of A. comosus N36 cultures.