Electrotransformation of Agrobacterium Tumefaciens with PSP' AP-VF2 and pCambia 1301 constructs

Abstract

Agrobacterium-mediated transformation is an effective plant genetic transformation method. The application of such method on microalgae transformation is extremely rare. In this study, the PSP'AP-VF2 construct which carries the gene encoding for antisense cDNA palmitoyl ACP-thioesterase and pCAMBIA 130 l construct which carries the reporter GUS gene were transferred into Agrobacterium tumefaciens strain LBA4404.