Electrotransformation of Agrobacterium Tumefaciens with p 35S-AP and pCambia 1304 constructs

Tan Lay Kim

dc.contributor.author	Tan Lay Kim
dc.date.accessioned	2018-01-02T07:33:56Z
dc.date.available	2018-01-02T07:33:56Z
dc.date.issued	2006
dc.identifier.uri	http://hdl.handle.net/123456789/7965
dc.description.abstract	Agrobacterium-mediated gene transfer is an important genetic transformation tool that has been used in plant genetic engineering to generate a wide variety of fertile transgenic plants. The p35S-AP construct carries antisense palmitoyl-ACP thioesterase cDNA driven by CaMV 35S promoter while the pCAMBIA 1304 construct carries genes encoding p-glucuronidase (uidA) and green fluorescent protein (gfp ). Both constructs were successfully electroporated into wild type A. tumefaciens strain LBA 4404 using electroporation apparatus, MicroPulser.	en_US
dc.language.iso	en	en_US
dc.publisher	Terengganu: Kolej Universiti Sains dan Teknologi Malaysia	en_US
dc.subject	Tan Lay Kim	en_US
dc.subject	LP 60 FST 3 2006	en_US
dc.title	Electrotransformation of Agrobacterium Tumefaciens with p 35S-AP and pCambia 1304 constructs	en_US
dc.type	Working Paper	en_US