Isolation and bioactivity screening of chemical constituents extracted from gracilaria changii

Abstract

Gracilaria changii has been extracted and fractionated to obtain the ethanol, diethyl ether, ethyl acetate, buthanol extracts and polysaccharide. In this research, isolation and separation of chemical compounds have been carried out by various solvent and chromatographic methods. The structure of these pure compounds have been determined by various spectroscopic methods such as infrared (IR), ultra-violet (UV), nuclear magnetic resonance (]H, l3C and 2D NMR), mass spectroscopy (MS) and by comparison with the data reported previously. The crude extracts, isolated pure compounds and synthetic compounds were subjected to the biological activities screening test such as antioxidant, antibacterial, cytotoxic assay and TLC bioautographic method for acetylcholinesterase inhibition assay.