Abstract:
More recent studies have demonstrated that conventional process such as
chromatography, electrophoresis, affinity purification, etc. widely used in protein
separation and purification now days. However, these processes are limited to
separation of protein solutes and shown that the purity of protein obtained
significantly less. Ultrafiltration has become an alternative technology for protein
separation over conventional bioseparation processes due to its high throughput of
product. A systematic study on the influence of different polymer concentration and
shear rate for ultrafiltration membrane in separating BSA protein was performed to
determine the best formulation and shear rate condition.
