Development of asymmetric bio-separation membranes for protein (bsa) separation

Abstract

More recent studies have demonstrated that conventional process such as chromatography, electrophoresis, affinity purification, etc. widely used in protein separation and purification now days. However, these processes are limited to separation of protein solutes and shown that the purity of protein obtained significantly less. Ultrafiltration has become an alternative technology for protein separation over conventional bioseparation processes due to its high throughput of product. A systematic study on the influence of different polymer concentration and shear rate for ultrafiltration membrane in separating BSA protein was performed to determine the best formulation and shear rate condition.