Abstract:
Cryptocoryne ciliata is the aquatic plant that easy to propagate in vitro, but no report
on its medicine value. Thus, the objectives of this study were to determine the anti
Vibrio bacteria activity and MIC (minimal inhibitory concentration) of extract for
antibacterial activity and also to determine the age of plant with highest antibacterial
activity. The anti-Vibrio bacteria activity of methanol and aqueous extract of
Cryptocoryne ciliata cultures were investigated. The in vitro plantlet was established
and proliferate on the MS media added with 3mg/l BAP for 30, 50 and 70 days of
cultivation time. The evaluation of antibacterial activity was done by using Kirby
Bauer method. The methanol extract of cultured plants have antibacterial against V
alginolyticus and V vulnificus with the diameter in range of 6mm to 9mm, but no
antibacterial activity when tested against V parahaemolyticus. The aqueous extracts
have no antibacterial activity against three tested bacteria. Among the three
cultivation time, 70-days have the highest inhibition zone following by 50 days and
30 days the lowest inhibition zone. These observations indicate of that the longer
cultivation time gave higher antibacterial activity.
