Anti vibrio bacteria activity of Cryptocoryne ciliate cultures

Abstract

Cryptocoryne ciliata is the aquatic plant that easy to propagate in vitro, but no report on its medicine value. Thus, the objectives of this study were to determine the anti Vibrio bacteria activity and MIC (minimal inhibitory concentration) of extract for antibacterial activity and also to determine the age of plant with highest antibacterial activity. The anti-Vibrio bacteria activity of methanol and aqueous extract of Cryptocoryne ciliata cultures were investigated. The in vitro plantlet was established and proliferate on the MS media added with 3mg/l BAP for 30, 50 and 70 days of cultivation time. The evaluation of antibacterial activity was done by using Kirby­ Bauer method. The methanol extract of cultured plants have antibacterial against V alginolyticus and V vulnificus with the diameter in range of 6mm to 9mm, but no antibacterial activity when tested against V parahaemolyticus. The aqueous extracts have no antibacterial activity against three tested bacteria. Among the three cultivation time, 70-days have the highest inhibition zone following by 50 days and 30 days the lowest inhibition zone. These observations indicate of that the longer cultivation time gave higher antibacterial activity.