Screening for lipase activitiy from md 019 bacteria isolated from coral mucus

Abstract

Lipases which are among the most important industrial enzymes have the ability to catalyze the breakdown of triacylglycerol to free fatty acids and glycerol. However, there are a few reports on the productions of lipase from marine bacteria. In this study, the screening for lipase activity from MDO 19 bacteria isolated from the mucus of Aeropora cervicornis coral at Bidong Island has been carried out. Several biochemical tests have been done manually to confirm the identification of the bacteria, based on their morphological and phenotypic characteristics. The results suggested that the bacteria is Erwinia sp. The presence of lipase enzyme in MD019 bacteria have been detected by halo zone on tributyrin agar. Several reaction parameters were studied to obtain the optimum reaction conditions for lipase activity in MDO 19 bacteria