Detection of fimbrial gene in organs infected by Pasteurella multocida B:2 using polymerase chain reaction (PCR) technique

Abstract

Rapid detection of Pasteurella multocida B: 2 are important due to the facts that these bacteria can cause serious outbreak that bring mortality to the affected animals. This study was conducted to detect the presence of fimbrial gene in rat's organs that infected with P. multocida B: 2 by using PCR technique. This technique was used to determine the ability of this technique to detect the presence of P. multocida B: 2 in the tissue organs. Three sets of fimbriae gene primers were used, Pair 1; EZl(F) and EZ2(R), Pair 2; EZ3(F) and EZ4(R) and Pair 3; EZ5(F) and EZ6(R). This study has successfully identified the presence of fimbrial gene in the infected tissue organs; lung, liver and kidney which suggested that there was a presence of P. multocida B: 2 in the tissue organs. Results indicated that all 3 set of primers have generated fimbriae gene of approximately 400-SOObp. The finding of this study has indicated that within 7 days of exposure, the bacteria has spread and colonize the tissue organs and it was suspected that the bacteria have spread through the blood stream of the infected animals. These studies have demonstrated that PCR technique using a fimbriae gene primers can be used as a diagnostic tool in detecting and identifying the presence of P. multocida B: 2 in the infected tissue organs.