Establishment tissue culture of Avicennia alba

Abstract

Mangroves are become extinction due to the development and economic values of the tree. The tissue culture or micropropagation technique can be an alternative method to conserve the mangrove especially Avicennia alba. The objectives of this study are to determine the suitable medium and phytohormone for tissue culture of Avicennia alba and its growth performance. Shoot tips and axillary bud were used as explants were obtained from mangrove area near at Universiti Malaysia Terengganu. The surface sterilization was done of varies strength of Clorox from 10% until 100% with varies immersion time from 10, 15 and 20 minutes. The best surface sterilization was obtained by using 100% Clorox with 20 minutes of immersion time which is 81 % of explants were sterile. The tissue culture technique was not established due to browning of explant.