Screening of different glyphosate resistance and susceptible biotypes of Eleusine indica with rapds

Abstract

In Malaysia, goosegrass (Eleusine indica) is a major threat in malaysian oil palm plantation that has evolved its glyphosate resistance since 1997 and its ED5o value is approximately 2 to 4 fold greater than that of susceptible biotype from the same region. Due to these problems, there is an interest involving molecular biology study to reveal the differences in DNA sequences of different goosegrass biotypes. Randomly Amplified Polymorphic DNA (RAPD) was used to screen genomic DNA of four different resistant biotypes (P6, P8, P20 and P24) and one susceptible biotype. Six RAPD primers (OPA-1, OPA-11, OPA-16, OPA-17, OPA-18 and OPA-19) were used for amplification. All together, six putative specific RAPD band were identified. Primer OPA-18, OPA-17 and OPA-19 produced specific bands with size of 450bp, 350bp and 500bp for biotype with 11-fold respectively. Thus, primer OPA-17 and OP A-11 produced specific bands with size of 400bp and 250bp for biotype with 8fold and 4-fold respectively. While, primer OPA-01 successfully produced specific band with size of 300bp for the susceptible biotype.