Serum Immunoglobulin G (igG) level in goats following exposure to live attenuated Pasteurella multocida B2

Abstract

Pasteurella multocida B2 is the causative agent of Haemorrhagic septicaemia (HS), a disease that infects cattle and water buffaloes in the region of Asia. One of the best methods to increase the protection against HS is by inducing the production of Immunoglobulin G (IgG). Thus, a study was carried out to determine serum IgG level in goats following exposure to live attenuated Pasteurella multocida B2. Nine clinically healthy goats of either sex aged 7-9 months old were divided into three groups. Goats in Group 1 (Negative Control) were the unexposed control; Goats in Group 2 (Positive Control) were subjected to double intranasal exposures to formalin-killed P. multocida B2 while goats in Group 3 (Treatment) were subjected to double intranasal exposures to live attenuated P. multocida B2. Enzyme-linked immunosorbent assay (ELISA) was subjected to the serum samples collected once every week from each group of goats to determine the level oflgG. As the results, significant differences (p < 0.05) oflgG were obtained among all the groups in the study. The serum IgG levels of goats exposed to live attenuated P. multocida B2 in Group 3 showed a higher level of lgG as a reaction of immune response following exposure throughout the study and peaked at Week 6 compared to the lgG level of goats in Group 1 and Group 2 detected throughout the study. It was concluded that live attenuated P. multocida B2 induced a higher level of IgG in goats in Group 3 compared to the lgG level of goats in Group 1 and Group 2.