Abstract:
Penicillin is useful for the treatment of bacterial infections. Microorganism such as
Acanthamoeba castelanii that inhibit the biosynthesis of the bacterial cell wall are
believe to produce penicillin antibiotics. Previous research by Chuah Huey Shuan had
identified twelve specific bands using primer combinations of IPNS-Fl/IPNS-R2,
IPNS-F2/IPNS-R2 and IPNS-F2/IPNS-RI with gradient PCR technique from ten
isolates of amoeba. The MAI fragment (850 bp) was selected for cloning, sequencing
and analyzing. The MAI fragment was reamplified using combination of IPNS
FI/IPNS-R2 primer with PCR technique.
