Cloning and sequencing of putative B-Lactam gene from selected amoeba isolate

Abstract

Penicillin is useful for the treatment of bacterial infections. Microorganism such as Acanthamoeba castelanii that inhibit the biosynthesis of the bacterial cell wall are believe to produce penicillin antibiotics. Previous research by Chuah Huey Shuan had identified twelve specific bands using primer combinations of IPNS-Fl/IPNS-R2, IPNS-F2/IPNS-R2 and IPNS-F2/IPNS-RI with gradient PCR technique from ten isolates of amoeba. The MAI fragment (850 bp) was selected for cloning, sequencing and analyzing. The MAI fragment was reamplified using combination of IPNS­ FI/IPNS-R2 primer with PCR technique.