Screening for lipase activity from MD030 bacteria islated from coral mucus

Abstract

There are very few environmental studies to date, focusing on lipase activities in marine microbes such as bacteria. In this research, the ability to produce lipase by marine bacteria MD030, isolated from coral mucus was investigated. The identification of the MD030 bacteria was confirmed by several biochemical tests based on their morphological and phenotypic characteristics. The results suggested that the bacteria are Moraxella sp. Prior to induction of lipase by the bacteria, three different media have been tested to determine the best culture medium: Nutrient Broth in Distilled Water, Nutrient Broth in Sea Water and ZoBell's modified medium. In this study, Nutrient Broth in Sea Water medium was chosen as the medium for lipase induction. The protein content of the crude enzyme was 1 SOµg/ml. The induction of lipase with olive oil as substrate was initiated with the preparation of pre-inoculum. Several reaction parameters influencing lipase activity such as incubation time, temperature and amount of substrate have been determined. There was no significant difference (p>0.05) between all incubation times tested.