Antifungal activities of free-living amoebae crude extracts againts Microsporum gypseum

Abstract

The objectives of this research were to investigate the antifungal activities of two extracts from free-living amoebae Acanthamoeba sp (AK and Pl) extracts on Microsporum gypseum by looking at their minimum inhibition concentration (MIC) values against the fungal species and identifying which amoeba extracts has the most potential in antifungal activities. In this study, extracts of two free-living amoebae obtained from Acanthamoeba sp (labelled as AK extract) and Acanthamoeba sp (labelled as Pl extract) were tested on Microsporum gypseum. Mgypseum is one of the pathogenic fungi that cause dermatophytosis, the infections of the skin, hair and nails. The amoeba extracts AK and Pl with various concentrations (6, 3, and l .5mg/ml) and (2, 1, and 0.5mg/ml) respectively were used, for the test on the fungus. Results obtained from this study indicated that all the extracts used have no antifungal activity against Mgypseum. There was no inhibition zone observed at all concentration of the extracts used, indicating that both amoeba extracts have no antifungal activities.