Screening for lipase activity by md 031 bacteria isolated from coral mucus

Abstract

This study was carried out to investigate the production of lipase by MD03 I bacteria isolated from coral mucus at Bidong Island, Terengganu. The identification of MD031 bacteria was confirmed by several biochemical tests based on the morphological and phenotypic characteristics. The results suggested that MD03 I bacteria is Bruce/la sp. The best medium for lipase induction has been determined between three different media: Zobell modified medium, nutrient broth in sea water and nutrient broth in distilled water. Zobell modified medium has been chosen as the best medium for culture growth. For the pre-inoculum preparation, it was determined that MD03 I bacteria took five hours to achieve OD reading of 0.5 at 600nm, which indicates the mid-log phase of bacteria. Different parameters were determined to find the optimum reaction conditions for lipase production: incubation time, temperature, and amount of substrate.