Abstract:
Acanthamoeba spp. was believed to possess vanous antimicrobial peptides and
proteins to combat bacterial growth inside their phagosomes. Tetracycline belongs to
a group of antibiotics that contain antimicrobial and pharmacokinetic properties.
Anhydrotetracycline oxygenase (anhydrotetracycline monooxygenase or AHTM) is
the enzyme that involve in the reaction of biosynthesis pathway of Streptomyces spp.
in the production of tetracycline. Previous studies had successfully identified six
putative fragments by using PCR technique. The putative fragment of AHTM5 was
selected and re-amplified and cloned into pGEM-T
Easy Vector. The putative recombinant colonies were selected for plasmid extraction. The presence of inserted
DNA was confirmed by using PCR technique. The complete nucleotide sequence
consists of 243bp while the deduced amino acids from the complete nucleotide
sequence are 81. The translated amino acid sequence of clone pAHTM5 shows 81 %85%
