Toxicity effect of Cymbopogon nardus crude extract on aedes aegypti (Diptera:culicidae)

Abstract

Botanical insecticides can be an alternative source for mosquito control because they constitute a potential source of bioactive compound and typically are free from harmful effects. This study was carrying out to search the alternative insecticides from the natural product such as herbal plant, which is non toxic to environment and biodegradable. The purposes of this study are to determine the toxicity effect of Cymbopogon nardus crude on third instar Aedes aegypti and to compare the toxicity of leaves and root extract. Leaves and root of C. nardus was extracted using 98.9% methanol and tested against third instar Ae. aegypti larvae (Diptera: Culicidea) under laboratory condition. Exposme was done for 24 hours in the concentration 4.0, 2.0, 1.0, 0.5 and 0.25mg/ml. The mean of LC5o of leaves was 2.3075 mg/ml and roots part was 2.1835 mg/ml. Two samples mean of LC5o showed no significant difference in both part of this plant (p > 0. 05). Developments of larval were completely inhibited by the treatment of crude extract. This study indicates that both parts of C. nardus have potential as larvacidal agents against Ae. aegypti.