Abstract:
The important of this study is to discover a novel antibiotic for pharmaceutical from
marine bacteria. The aim of this study is to determine the antibacterial activity of an
extracellular protein produced by coral-mucous associated bacteria activity. Culture
coral-mucous associated bacteria from previous study were used as inoculums. The
drop test assay and overlaid technique were used to screen antibacterial activity
against Escherichia coli. From a total of 21 strains, seven of them were shown an
antibacterial activity, which were MD008 (Micrococcus varians), MDOl l (Cadacea
darisae), MD012 (Serratia plymuthica), MD018 (Citrobacter younge), MD019
(Erwinia herbicola), MD022 (Klebsiella pneumonia subspecies) and MD024
(Aeromonas cariae). Strain MD018 and MD019 were shown the highest
antibacterial activity on day 7 of incubation. Drop test assays, was revealed that
extracellular protein from MD019 was responsible for antibacterial activity.
Minimum inhibition concentration from MD019 was 10.6µg/ml with 10mm
diameter of clear zone. Few marine bacteria source of bioactive component with
antibacterial activity.
