Preliminary study on antibacterial activity of extracellular protein from coral mucous associated bacteria

Abstract

The important of this study is to discover a novel antibiotic for pharmaceutical from marine bacteria. The aim of this study is to determine the antibacterial activity of an extracellular protein produced by coral-mucous associated bacteria activity. Culture coral-mucous associated bacteria from previous study were used as inoculums. The drop test assay and overlaid technique were used to screen antibacterial activity against Escherichia coli. From a total of 21 strains, seven of them were shown an antibacterial activity, which were MD008 (Micrococcus varians), MDOl l (Cadacea darisae), MD012 (Serratia plymuthica), MD018 (Citrobacter younge), MD019 (Erwinia herbicola), MD022 (Klebsiella pneumonia subspecies) and MD024 (Aeromonas cariae). Strain MD018 and MD019 were shown the highest antibacterial activity on day 7 of incubation. Drop test assays, was revealed that extracellular protein from MD019 was responsible for antibacterial activity. Minimum inhibition concentration from MD019 was 10.6µg/ml with 10mm diameter of clear zone. Few marine bacteria source of bioactive component with antibacterial activity.