Abstract:
The microalgae Chlorella sp. is used as a nutrient-dense foods and sources of fine
chemicals such as lipid, protein, chlorophyll, vitamins and mineral that improve
health. The genetic transformation was conducted to enhance the polyunsaturated
fatty acids which contribute for human health. Chlorella sp. was cultured successfully
and used to prepare electrocompetent cells.The objectives of this study were to
transform the Chlorella sp. with PSP-AP2 circular construct and select the putative
recombinant Chiarella cells. The plasmid DNA of PSP-AP2 construct was extracted
from E.coli. the presence of the PSP-AP2 construct was detected with two primer
combinations. The primers PTEVF1/PTEVR2 amplified a band of 617 bp while the
primers PTEVF1/ProVF2 amplified a band of 1047 bp.
