Electroporation of Chlorella sp. with psp-Ap 2 circular construct

Abstract

The microalgae Chlorella sp. is used as a nutrient-dense foods and sources of fine chemicals such as lipid, protein, chlorophyll, vitamins and mineral that improve health. The genetic transformation was conducted to enhance the polyunsaturated fatty acids which contribute for human health. Chlorella sp. was cultured successfully and used to prepare electrocompetent cells.The objectives of this study were to transform the Chlorella sp. with PSP-AP2 circular construct and select the putative recombinant Chiarella cells. The plasmid DNA of PSP-AP2 construct was extracted from E.coli. the presence of the PSP-AP2 construct was detected with two primer combinations. The primers PTEVF1/PTEVR2 amplified a band of 617 bp while the primers PTEVF1/ProVF2 amplified a band of 1047 bp.