Abstract:
Goosegrass (Eleusine indica) is a monocot weed in the Poaceae family and distributes
in many areas through out the world. This study was carried out to screen the different
biotypes of Eleusine indica with RAPD and to identify RAPD markers for each
resistance levels of goosegrass. Five populations of Eleusine indica from various
places of plantation area in Kedah and Perak were screened with glyphosate-sprayed
at different level of doses along with a standardized condition in University Malaysia
Terengganu greenhouse for two weeks. The results revealed that five different
biotypes; consist of susceptible, 2-folds, 4-folds, 8-folds and the highest resistant level
observed was 11-folds. These populations underwent RAPD screening of six primers;
OPA-05, OPA-06, OPA-12, OPA-14, OPA-15 and OPA-20 resulting in 8 different
random amplified polymorphic DNA markers. This study clarified the use of
polymorphism exhibited by random amplified polymorphic DNA of Eleusine indica
in generating a particular genetic marker for each biotypes and totally beneficial for
weed management problem in the future.
