Screening of different glyphosate resistance and susceptible biotypes of Eleusine indica (L.) Gaertn with Rapds

Abstract

Goosegrass (Eleusine indica) is a monocot weed in the Poaceae family and distributes in many areas through out the world. This study was carried out to screen the different biotypes of Eleusine indica with RAPD and to identify RAPD markers for each resistance levels of goosegrass. Five populations of Eleusine indica from various places of plantation area in Kedah and Perak were screened with glyphosate-sprayed at different level of doses along with a standardized condition in University Malaysia Terengganu greenhouse for two weeks. The results revealed that five different biotypes; consist of susceptible, 2-folds, 4-folds, 8-folds and the highest resistant level observed was 11-folds. These populations underwent RAPD screening of six primers; OPA-05, OPA-06, OPA-12, OPA-14, OPA-15 and OPA-20 resulting in 8 different random amplified polymorphic DNA markers. This study clarified the use of polymorphism exhibited by random amplified polymorphic DNA of Eleusine indica in generating a particular genetic marker for each biotypes and totally beneficial for weed management problem in the future.