Establishment of tissues culture of Melalwuca leucadendron

Abstract

The purpose of this study is to determine the best and suitable hormone for growth and proliferation of calli of Melaleuca /eucadendron. The best sterilization treatment for leaves was 20% Chlorox (v/v) and immersed for 30 minutes and the sterilization for stem not obtained yet. Initiation calli from young leaves culture was established in Murashige and Skoog (MS) medium added with picloram (1, 2, 3, 5, 7 and 10mg/L) or 2, 4-Dichlorophenoxyacetic acid (1, 2 and 3mg/L) or dicamba (1, 3, 5 and 7mg/L) alone and combination of 2, 4-D with kinetin at ratio of 1:1, 2:1 and 3:lmg/L or picloram with kinetin at ratio of 1: 1 and 2: lmg/L or picloram with BAP at ratio of 5:1, 7:1 and 10:lmg/L. Calli was produced in 5.0mg/L picloram, 3.0 or 5.0mg/L dicamba. Subculture of calli from 5.0mg/L picloram was died after two weeks but subculture of calli from 3.0mg/L dicamba form root after two weeks. Dicamba was more effective and suitable hormone compare to other hormones for calli induction of young leaves of M /eucadendron.